![](https://parts.igem.org/images/partbypart/icon_coding.png)
Part:BBa_K2398013:Design
p15A origin with Ampicillin resistance for application in Phage-assisted continous evolution (PACE)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1
Illegal BglII site found at 2160 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 185
Illegal AgeI site found at 509 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1214
This was designed for the use with the cloning standard of the iGEM Team Heidelberg 2017 (http://2017.igem.org/Team:Heidelberg/RFC). It is meant for the usage as building block for accessory plasmids in the context of phage assisted continuous ecolution [1] or related systems [2].
The part is fully compatible with RFC10, as well as with Golden Gate assembly
Source
E. coli, pSB1A3, with modifications.
References
[1] Esvelt, Kevin M.; Carlson, Jacob C.; Liu, David R. (2011): A system for the continuous directed evolution of biomolecules. In: Nature 472 (7344), S. 499–503. DOI: 10.1038/nature09929.
[2]Brödel, Andreas K.; Jaramillo, Alfonso; Isalan, Mark (2016): Engineering orthogonal dual transcription factors for multi-input synthetic promoters. In: Nature communications 7, S. 13858. DOI: 10.1038/ncomms13858.